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dmem high glucose medium with hepes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dmem high glucose medium with hepes
    Dmem High Glucose Medium With Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+high+glucose+medium+with+hepes/HEPES/us12551514-498-7-13
    Average 99 stars, based on 1 article reviews
    dmem high glucose medium with hepes - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Combination of non-viable cells of
    Article Snippet: Bone Marrow cells were then resuspended in RPMI 1640 media supplemented with 2 mM GlutaMax (ThermoFisher, Cat. No. 30-2001), 10% HI FBS (Avantor, Cat. No. 1500-500H), 1% P/S (Gibco, Cat. No. 15140-122), 50 ng/ml GM-CSF (PeproTech, Cat. No. 300-03), 25 ng/mL of IL-4 (PrepoTech, Cat. No. 200-04) and cultured in 96 well plate (3×104 cells/well) at 37° C. with 5% CO2 for 48 h. Dendritic cells (DCs) differentiation continued after refreshing half of the media for 24 h. Next, cell media was completely refreshed, and cells were cultured for another 72 h to complete DCs differentiation. .. MB49 cells (2.5×106 cells) were cultured in DMEM High Glucose medium with HEPES (ThermoFisher, Cat. No. 12430054), 10% HI FBS, and 1% P/S in T-25 flasks and treated with COMPOSITION-002 (0, 0.05, 0.2, 0.8, 1.6, and 3.2 KE/mL) for 24 h. Dinaciclib (1 μM) (Tocris R&D, Cat. No. 7336) was used as positive control. .. After treatment, cells were resuspended, washed to remove COMPOSITION-002, and stained with VybrantTM DiO Cell-Labeling Solution (ThermoFisher, Cat. No. V22886) for 20 min at 37° C. Pre-labeled MB49 cells and DCs were co-cultured in 96 well plates at 2:1 ratio (3×104 DCs: 1.5×104 MB49 cells) for 24 h using DC medium: MB49 medium ratio at 1:1.

    Positive Control:

    Article Title: Combination of non-viable cells of
    Article Snippet: Bone Marrow cells were then resuspended in RPMI 1640 media supplemented with 2 mM GlutaMax (ThermoFisher, Cat. No. 30-2001), 10% HI FBS (Avantor, Cat. No. 1500-500H), 1% P/S (Gibco, Cat. No. 15140-122), 50 ng/ml GM-CSF (PeproTech, Cat. No. 300-03), 25 ng/mL of IL-4 (PrepoTech, Cat. No. 200-04) and cultured in 96 well plate (3×104 cells/well) at 37° C. with 5% CO2 for 48 h. Dendritic cells (DCs) differentiation continued after refreshing half of the media for 24 h. Next, cell media was completely refreshed, and cells were cultured for another 72 h to complete DCs differentiation. .. MB49 cells (2.5×106 cells) were cultured in DMEM High Glucose medium with HEPES (ThermoFisher, Cat. No. 12430054), 10% HI FBS, and 1% P/S in T-25 flasks and treated with COMPOSITION-002 (0, 0.05, 0.2, 0.8, 1.6, and 3.2 KE/mL) for 24 h. Dinaciclib (1 μM) (Tocris R&D, Cat. No. 7336) was used as positive control. .. After treatment, cells were resuspended, washed to remove COMPOSITION-002, and stained with VybrantTM DiO Cell-Labeling Solution (ThermoFisher, Cat. No. V22886) for 20 min at 37° C. Pre-labeled MB49 cells and DCs were co-cultured in 96 well plates at 2:1 ratio (3×104 DCs: 1.5×104 MB49 cells) for 24 h using DC medium: MB49 medium ratio at 1:1.



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